Molecular cloning of F11 fimbriae from a uropathogenicEscherichia coliand characterization of fimbriae with polyclonal and monoclonal antibodies

Identification and Purification of F11 Fimbriae on Avian Pathogenic Escherichia coli

Determination of P (F11) and F1 fimbriae of Escherichia coli isolated from avian cellulitis

Avian cellulitis has been induced by virulent strains of E. coli. The purpose of this study was to determine P (F11) and F1 fimbriae of 90 E. coli isolates of avian cellulitis. Isolates were subjected to six consecutive passages on solid and static broth for expression of fimbriae. Five (5.5%) isolates from O1 serogroup, showed a mannose-resistant haemagglutination MRHA pattern when grown on so...

Characterization of Haemophilus influenzae type b fimbriae.

We confirmed that the fimbriae of Haemophilus influenzae type b conferred hemagglutinating activity (HA) towards human erythrocytes, and erythrocytes of certain other species. Most (17/25) cerebrospinal fluid isolates lacked detectable HA on direct testing, but selective enrichment for fimbriation (f+) indicated that 22 of 25 strains could produce these surface structures. HA was unchanged from...

Purification and characterization of fimbriae isolated from Bordetella pertussis.

Fimbriae were detached from Bordetella pertussis by mechanical shearing and purified by successive precipitations with ammonium sulfate, phosphate buffer (pH 6.0), and magnesium chloride. In each of these purification steps, the fimbriae aggregated into bundles as seen by electron microscopy. These aggregates could be disaggregated at pH 9.5. By electron microscopy, the purified fimbriae appear...

Comparison of type 2 and type 6 fimbriae of Bordetella pertussis by using agglutinating monoclonal antibodies.

Two types of fimbriae have been identified on the pathogenic gram-negative organism Bordetella pertussis. Monoclonal antibodies to these fimbriae were produced to better understand the role of fimbriae as serotype-specific agglutinogens and to investigate the antigenic relationship between these fimbriae. Three monoclonal antibodies were identified that specifically agglutinated B. pertussis ce...